Figure 4: Coomassie-blue-stained SDS gel and Western blot analysis of AtFAAH probed with anti-HIS antibodies and kinetic characterization of recombinant AtFAAH with 100 μM MDPD. | Scientific Reports

Figure 4: Coomassie-blue-stained SDS gel and Western blot analysis of AtFAAH probed with anti-HIS antibodies and kinetic characterization of recombinant AtFAAH with 100 μM MDPD.

From: A chemical genetic screen uncovers a small molecule enhancer of the N-acylethanolamine degrading enzyme, fatty acid amide hydrolase, in Arabidopsis

Figure 4

The epitope-tagged, recombinant AtFAAH protein used in the assays is predicted to be ~70 kDa (arrow). 1. crude protein extract; 2. supernatant fraction; 3. flow through; 4. eluted fraction (AtFAAH); 5, Western blot of eluted fraction; MM, Molecular marker. Initial velocities were plotted at increasing concentrations of [1-14C]-NAE 12:0 with and without MDPD. Reactions were initiated by the addition of AtFAAH. Catalytic parameters were calculated from these initial velocity data. Data points represent means ± S.D. of triplicate assays.

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