Figure 5: Amidohydrolase (AHase) assays on total seedling protein extracts and in vivo [1-14C]-NAE 12:0 depletion assays.

(a) Increased AHase activity in crude protein extracts with MDPD. (b) [1-14C]-NAE 12:0 is depleted in liquid medium containing wild-type seedlings. Note that depletion of radiolabeled NAE 12:0 is faster in the presence of MDPD. Student’s t-test (**indicates statistically significant differences, p < 0.05).