Figure 5: Effect of MSCs and LY294002on the cell viability, cell injury, protein expressions and Annexin V-FTIC/PI double-staining flow cytometry of myoglobin-induced apoptosis in HK-2 cells. | Scientific Reports

Figure 5: Effect of MSCs and LY294002on the cell viability, cell injury, protein expressions and Annexin V-FTIC/PI double-staining flow cytometry of myoglobin-induced apoptosis in HK-2 cells.

From: Biological Membrane-Packed Mesenchymal Stem Cells Treat Acute Kidney Disease by Ameliorating Mitochondrial-Related Apoptosis

Figure 5

(a) The MTT assay showed the cell viability of HK-2 cells. (b) Supernatant LDH release tests indicated cell injury of HK-2. (c) p-Akt, Bad, Bcl-xl, Cytochrome c, cleaved caspase-9 and cleaved caspase-3 were measured by western blot. (d) Cell apoptosis was evaluated by AnnexinV-FTIC/PI double-staining flow cytometry. Note: *p < 0.05 versus the Con group, #p < 0.05 versus the Mb group, **p < 0.05 versus the MSC group n = 6.

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