Figure 10: MoVrp1 is involved in autophagy. | Scientific Reports

Figure 10: MoVrp1 is involved in autophagy.

From: MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae

Figure 10

(A) Organelles and autophagic bodies were observed in vacuoles under the starvation condition. Arrowheads represent the autophagic bodies in vacuoles. Bars = 1 μm. (B) Guy11 and ∆Movrp1 mutant expressing GFP-Atg8 were grown in liquid CM medium at 28 °C for 10 h, and shifted to liquid MM-N medium with 2 mM PMSF for 4 h. Hyphae were examined by DIC or epifluorescence microscopy. (C) Statistical analysis of the vacuoles contained GFP in hyphae. The experiments were repeated three times with triple replications. Error bars represent standard deviation from the mean, and t test analysis is shown with *p < 0.01 versus the wildtype Guy11. (D) GFP-Atg8 proteolysis assays of Guy11 and ∆Movrp1 mutant. Mycelia were cultured at 28 °C for 10 h in liquid CM medium and shaken at 150 rpm. Autophagy was induced after 8 h of nitrogen starvation. Mycelia were harvested and mycelia extracts were analyzed by western blot using anti-GFP.

Back to article page