Figure 5: Deletion of MoVRP1 altered the distribution of chitin. | Scientific Reports

Figure 5: Deletion of MoVRP1 altered the distribution of chitin.

From: MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae

Figure 5

(A) Disruption of MoVRP1 altered the chitin distribution on the cell wall. Hyphae of the wildtype Gyu11, ∆Movrp1#54, ∆Movrp1#105 and the complemented strain were stained by 10 μg/mL CFW for 2 min and then photographed. (B) Expression levels of seven CHS genes in the wildtype Guy11 and ∆Movrp1 mutant. (C) Protoplasts of the wildtype Guy11 and ∆Movrp1 mutant were counted when the mycelia were treated 30, 60 and 90 min with the solution of cell wall chitinase. (D) Western blot analysis of phosphorylation level of Mps1. The activation was detected with an anti-MAPK antibody and an anti-Actin was used as a positive control. All experiments were repeated three times with triple replications. Error bars represent standard deviation from the mean, and t test analysis is shown with *p < 0.01 versus the wildtype Guy11.

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