Figure 9: MoVrp1 is involved in extracellular laccase and peroxidase activities. | Scientific Reports

Figure 9: MoVrp1 is involved in extracellular laccase and peroxidase activities.

From: MoVrp1, a putative verprolin protein, is required for asexual development and infection in the rice blast fungus Magnaporthe oryzae

Figure 9

(A) The discoloration of CR was tested on CM plates. Strains were inoculated on CM plates containing 200 μg/ml of Congo red and discoloration was observed on day 7 at 28 °C. (B) Laccase activities of the wildtype Guy11, ∆Movrp1#54, ∆Movrp1#105 and complemented strain were tested on CM plates containing 0.2 mM ABTs and photographed after 3 days incubate at 28 °C. (C) Extracellular peroxidase activities measured by ABTs oxidizing test under H2O2 supplemented condition. (D) Laccase activities measured by ABTs oxidizing test without H2O2. (E) Expression levels of 16 laccase- or peroxidase-encoding genes in the wildtype Guy11 and the ∆Movrp1 mutant. All experiments were repeated three times with triple replications. Error bars represent standard deviation from the mean, and t test analysis is shown with *p < 0.01 versus the wildtype Guy11.

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