Figure 5: Neural stem cell differentiation in different media compositions. | Scientific Reports

Figure 5: Neural stem cell differentiation in different media compositions.

From: Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D

Figure 5

(a) Scanned images of neurospheres stained for markers of neuronal (βIII-tubulin) and glial (GFAP) differentiation, progenitors (SOX-2) and morphology (H&E). Different media conditions are arranged in columns, while the markers are arranged in rows. Scale bar 500 μm. (b) Tukey boxplot of the area positive for GFAP (red, left box) and βIII-tubulin (blue, right box) compared to the total area of the sphere. (c) Tukey boxplot of the number of nuclei positive for SOX-2 compared to the total number of nuclei in the spheroid. For (b) and (c) the line represents the median percentage, the box is formed by the first and third quartile, whiskers are 1.5 times the quartiles and points represent outliers. (d) to (f)- Graphs of the magnitude of the difference between the various differentiation media and NSC medium for βIII-tubulin (d), GFAP (e) and SOX-2(f). Dots (d) and squares (e,f) represent the mean difference, while the error bars are 95%CIs from the ANOVA analysis with Dunnet’s multiple comparison test follow-up.

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