Figure 6: Effects of etoposide on the proliferation and neuronal differentiation of fetal brain neurospheres.

(a) Ratio of Ki-67 positive nuclei (in percent) as a function of increasing etoposide concentration. (b) Relative area positive for βIII tubulin (%). Dots are mean values derived from 3 sections from 3 experimental repeats, error bars are SDs. Four parameter-dose response curves were fitted to the data. (c) Representative images of untreated spheroids (left) and spheroids at increasing concentrations of etoposide stained for Ki-67(top) and βIII-tubulin (bottom). Scale bar 500 μm.