Table 1 Table of differentiation media composition.
Basal media (100 mL) | Additions | Short name |
|---|---|---|
DMEM (47.5 mL) | EGF(20 ng/mL) and FGF(10 ng/mL) | NSC |
F12 (47.5 mL) | — | Basal |
B27 (2 mL) | Retinoic acid (300 ng/mL) | RA |
N2 (1 mL) | NGF (10 ng/mL) | NGF |
Heparin (5 μg/mL) | diBut-cAMP (500 μg/mL) | diBut-cAMP |
Forskolin (4.1 μg/mL) | Forskolin | |
Fetal bovine Serum (5% vol/vol) | Serum/FBS | |
Cultrex BME (250 μg/mL) | BME | |
BDNF (50 ng/mL) | BDNF | |
NT-4 (10 ng/mL) | NT-4 |
