Table 3 List of antibodies (Ab) used in the IHC experiments.
Antibodies | Dilution | Sources | Cat. no |
|---|---|---|---|
Primary | |||
Mouse monoclonal anti-Ki-67 Ab (MIB-1) | 1:150 | DAKO | M7240 |
Mouse monoclonal anti-βIII-tubulin Ab (TU-20) | 1:500 | Abcam | ab7751 |
Rabbit polyclonal anti-GFAP Ab | 1:1000 | Abcam | ab7260 |
Rabbit polyclonal anti-SOX-2 Ab | 1:5000 | Abcam | ab97959 |
Secondary | |||
Polyclonal rabbit anti-mouse HRP | 1:300 | DAKO | P0161 |
Polyclonal swine anti-rabbit biotinylated | 1:300 | DAKO | E0353 |
