Figure 1: Differential apoptotic effects of CAPP on different types of human skin cells. | Scientific Reports

Figure 1: Differential apoptotic effects of CAPP on different types of human skin cells.

From: Mechanistic insights into the impact of Cold Atmospheric Pressure Plasma on human epithelial cell lines

Figure 1

(A) Primary keratinocytes, fibroblasts, HaCaT, HCT-116 and SK-MEL-28 cells were exposed to plasma treatment (He, He-O2 and He-N2) for 5 min with 1 hr post-treatment storage. Cells were stained with Annexin V-FITC and PI and analyzed by flow cytometry 24 hr after plasma treatment. Percentage of apoptotic cells (Annexin-PI positive) was shown by histogram, staurosporine treatment was used as a control of necrotic cells. The data shown is representative of three separate cultures. (B) Effect of plasma activated liquid (PAL) on cell viability. HaCaT cells were exposed to PAL for 1 hr (PBS treated for 5 min with He plasma, 580 μM H2O2 and 300 μM NO2− measured in the PAL); He-O2 plasma (40 μM H2O2 and 50 μM NO2− measured in the PAL) and He-N2 plasma (390 μM H2O2 and 300 μM NO2− measured in the PAL) or to a mix of 580 μM hydrogen peroxide and 300 μM mM NO2−, then, stained with Annexin V-FITC and PI, and analyzed by flow cytometry 24 hr after treatment. (C) For the same CAPP treatment active caspase-3 was analyzed by flow cytometry. (D) Cleaved PARP was assessed by western blot analysis. PARP cleavage was express as a % of total PARP using Jurkat cells as a control to assess native PARP. Data, mean ± SEM from three independent cultures **P < 0.01.

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