Figure 4: AmiE involvement in production of QS molecules.

(A) AHL production by the indicated P. aeruginosa strains. C. violaceum CV026 was used as a biosensor for AHL detection. An overnight culture of C. violaceum CV026 was mixed in a ratio 1:70 (v/v) with LB medium agar and each P. aeruginosa strain was spotted on the agar medium and incubated during 24 h at 37 °C. Three independent experiments have been realized resulting in the same pattern. (B) Comparison of C₄-HSL (left) and of 3-oxo-C₁₂-HSL (right) production by P. aeruginosa AmiE⁺ and PA14 EV at 3 h, 5 h and 24 h of growth. (C) Comparison of PQS production by P. aeruginosa AmiE⁺ and PA14 EV at 5 h and 24 h of growth. Data are the mean of three independent experiments. ^★★★P < 0.001; ^★★P < 0.01; NS: Not significantly different.