Figure 5: AmiE involvement in toxin production and cytotoxic activity.
From: The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence

(A) Relative amounts of HCN/CN− in supernatants of P. aeruginosa strains culture. The mean HCN/CN− level in the control was 1,834 ± 134 μg.l−1. (B) Relative amounts of pyocyanin in supernatants of P. aeruginosa strains culture. (C) Involvement of AmiE in P. aeruginosa cytotoxicity towards A549 lung cells. The measurement of LDH was done after 6 h of contact with PA14 WT, ΔamiE, PA14 EV and AmiE+ strains. All data are the mean of three independent experiments. ★★★P < 0.001.