Table 1 Effect of CGA-JK3 on LPS-induced production of TNF-α, IL-1α or IFN-β in macrophages.

From: Caffeic Acid Cyclohexylamide Rescues Lethal Inflammation in Septic Mice through Inhibition of IκB Kinase in Innate Immune Process

Treatment

TNF-α (pg/ml)

IL-1α (pg/ml)

IFN-β (pg/ml)

None

306 ± 87

43 ± 15

117 ± 29

LPS alone

1867 ± 104#

429 ± 46#

735 ± 68#

LPS + CGA-JK3 (1 μM)

1704 ± 161

375 ± 27

732 ± 54

LPS + CGA-JK3 (3 μM)

1430 ± 96*

353 ± 51

701 ± 22

LPS + CGA-JK3 (10 μM)

1005 ± 125*

226 ± 38*

818 ± 54

LPS + CGA-JK3 (30 μM)

415 ± 79*

125 ± 26*

738 ± 47

  1. Peritoneal macrophages were isolated from C57BL/6 J mice. The cells were stimulated with LPS (100 ng/ml) for 24 h in the presence of CGA-JK3. Aliquots of the culture supernatants were loaded onto ELISA kits to determine protein levels of TNF-α, IL-1α or IFN-β. Data are mean ± SEM from three independent experiments using the average values of triplicate in each experiment. #P < 0.05 vs. media alone-added group. *P < 0.05 vs. LPS alone-stimulated group.
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