Figure 4: Thermostability and specific activity of TK variants from two strategies.
From: Two strategies to engineer flexible loops for improved enzyme thermostability
Thermostability was examined by incubating 100 μL 0.1 mg/mL enzymes in triplicate under 60 °C for 1 h. Enzyme activity was measured at 22 °C with 50 mM GA, 50 mM HPA, in 50 mM Tris-HCl, pH 7.0 before and after heating. Specific activity in the plot was the initial activity measured before heating.
