Table 2 Characteristics of wild-type and mutant TKs.
From: Two strategies to engineer flexible loops for improved enzyme thermostability
Specific activity 22 °C | (μmol mg−1min−1) 60 °C | Tm (°C) | Half life t1/2 (min) | Km (mM) | kcat (s−1) | kcat/Km (s−1 M−1) | |
|---|---|---|---|---|---|---|---|
WT | 48.8 (0.4) | 89.8 (1.4) | 70.4 (0.3) | 22.9 | 20.5 (1.3) | 62.3 (2.4) | 3039 |
D143K | 46.7 (1.6) | — | 69.9 (0.4) | 25.2 | 16.1 (1.2) | 48.1 (3.2) | 2988 |
I189H | 1.9 (0.1) | 40.9 (1.1) | 72.3 (0.2) | 56.8 | 18.0 (0.8) | 1.5 (0.03) | 833 |
H192P | 57.3 (1.0) | 158.0 (3.7) | 74.0 (0.2) | 43.9 | 18.0 (1.2) | 65.5 (1.9) | 3639 |
A282P | 22.3 (0.5) | 89.3 (1.1) | 74.9 (0.2) | 36.1 | 17.6 (2.1) | 45.5 (1.5) | 2585 |
H192P/A282P | 59.9 (1.4) | 254.7 (2.3) | 75.0 (0.1) | 63.0 | 23.3 (1.4) | 81.2 (4.2) | 3485 |
