Figure 1: Differentiation of patient-specific iPSCs into hepatocyte-like cells (HLCs).

(a) A four-step differentiation protocol spanning 20 days was used to derive HLCs from iPSCs. (b) Phase contrast images of iPSC-derived HLCs after 20 days of hepatic differentiation. Scale bar = 100 μm. (c) Percentage of albumin+ cells in different patient-specific iPSC-derived HLCs on day 20. Data are average ± s.e.m of at least 3 independent differentiation experiments initiated at distinct timepoints. *p < 0.05 compared to primary human hepatocytes (PHP) control (Bonferroni t-test).