Figure 3: NHT and HT-iPSC derived HLCs exhibit differential in vitro hepatotoxicity to PZ but not to acetaminophen. | Scientific Reports

Figure 3: NHT and HT-iPSC derived HLCs exhibit differential in vitro hepatotoxicity to PZ but not to acetaminophen.

From: Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity

Figure 3

(a) Cell viability of PHP(i) and HLCs (ii–vi) measured after 24 hours incubation with different concentrations of PZ. HLCs derived from iPSCs of patients without clinical PZ toxicity, NHT1 and NHT2 (ii,iii). HLCs derived from iPSCs from patients with clinical hepatotoxicity HT1, HT2 and HT3 (iv–vi). (b) Cell viability of PHP (i) and patient-specific HLCs (ii–vi) measured by MTS assay after 24 hours incubation with different concentrations of acetaminophen (APAP). In (a and b) data are means ± s.e.m. of 3 independent differentiation experiments. (c) Tabulation of pair-wise comparisons of cell viability measured by MTS assay of different patient-specific HLCs after treatment with 100 μM PZ. (d) Pair-wise comparisons of cell viability of HLCs measured by ATP assay. p-values of paired t-tests are indicated. Significantly different pairs based on Bonferroni corrected t-test (p < 0.05) are labeled red. Non-significantly different pairs are labeled blue.

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