Figure 7: Absence of Rer1 reduces Nav protein levels.
From: The sorting receptor Rer1 controls Purkinje cell function via voltage gated sodium channels
(a) Brain lysates of Rer1Δbrain (ko) and control littermates (wt) of different ages as indicated were separated by SDS-PAGE, blotted and probed with indicated antibodies. In the Cav2.1 blot the irrelevant lane #7 was removed and the two last lanes flipped vertically, to match loading of the other gels. See full-length blot in the supplemental Fig. 5. P38, synaptophysin. (b) De-glycosylation of Nav1.1 and 1.6 indicates maturation status of the different bands. Brain lysates of P15 wt mice were subjected to de-glycosylation with endoglycosidase H (H) or PNGaseF (F) and processed for Western Blotting with Nav1.1 and 1.6 antibodies. Im, immature (endoH sensitive); m, mature (endoH resistant); degl, de-glycosylated Nav1.1 or 1.6 after PNGase F digest. In a, b cropped blots are shown, full-length blots are shown in supplemental Figs 5,6.
