Figure 3: ZP3 might function in germinal vesicle breakdown in multiple ways.
From: ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis
(A,B) To identify ZP3-interacting proteins, Immunoprecipitation was done with control IgG or ZP3 antibody in lysate of ZP-free GV oocytes and then samples were separated on a SDS-PAGE gel and subsequently subject to silver staining. Presumable bands distinct from control (blue arrow head pointed) were submitted for MALDI-TOF-MS. Besides ZP3 itself, Ptprk (protein tyrosine phosphatase, receptor type K), Aipl1 (aryl hydrocarbon receptor-interacting protein-like 1) and Diaph2 (diaphanous related formin 2) were identified as major ZP3-interacting proteins. An working model was established based on reference search & software prediction: ZP3 might regulate Ptprk, which could regulate Akt activity by phosphorylation; ZP3 might regulate Aip1, which could regulate lamin farnesylation; ZP3 might regulate Diaph2, which could regulate actin cytoskeleton organization.
