Figure 6: Compound 1 inhibits cell growth in a dose- and time-dependent manner.

(a) Exponentially growing HT-29 cells were treated with increasing doses of Rosiglitazone or 1 (1, 5 and 10 μM) for 24 and 48 h, harvested and counted. Data are the mean ± SD of three independent experiments performed in duplicate giving similar results. Significance is indicated as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001. (b) HT29 cells were seeded in 24-well in order to reach the density of cells/cm² in standard conditions (10⁵ cells). After 24 h, cells were treated with GW9662 (10 μM) for six hours, exposed to Rosiglitazone or 1 (10 μM) for 24 and 48 h, collected and counted. The experiments were performed in triplicate and the data expressed as mean ± SD. Significance is indicated as **p ≤ 0.01 and ***p ≤ 0.001.