Figure 1: Highly expressed CKIP-1 together with downregulated Smad-dependent BMP signaling and decreased bone formation in GIO. | Scientific Reports

Figure 1: Highly expressed CKIP-1 together with downregulated Smad-dependent BMP signaling and decreased bone formation in GIO.

From: Targeting osteoblastic casein kinase-2 interacting protein-1 to enhance Smad-dependent BMP signaling and reverse bone formation reduction in glucocorticoid-induced osteoporosis

Figure 1

(a) The intra-osseous CKIP1 mRNA (left) and protein (right) levels in patients from GIO and Control groups, respectively. (b) The intra-osseous total Smad1/5, pSmad1/5, Smurf1 and MEKK2 protein in patients from GIO and Control groups. Upper pannel: the quantitative data. Lower pannel: the representative electrophoretic bands (cropped). (c) The intra-osseous OCN mRNA level and the serum BALP level in patients from GIO and control groups. (d) Representative reconstructed micro-CT images for the trabecular architecture at the left tibia from the mice in each group. Scale bar: 100um. (e) The values of micro-CT parameters (BMD, BV/TV) in each group. (f) The values of bone histomorphometric parameter (MAR) in mice from each group. (g) The intra-osteoblast CKIP-1 mRNA levels in OCN + cells isolated by LCM in each group. (h) Representative immunofluorescence images for the expression of CKIP-1 (left three panel) and p-Smad1/5 (right three panel) in OCN + cells at the cryosection of proximal tibia from the mice in each group. Merged images with DAPI staining showed cells co-staining of CKIP-1 and OCN (arrow, left three lower panel), and p-Smad1/5 and OCN (arrow, right three lower panel). Scale bar = 50 μm. Note: Full-length electrophoretic bands are presented in Supplementary Figure 6. All data are the mean ± s.d. In the study with human specimens, the relative mRNA and protein levels are normalized to the mean value of the Control group. Human GAPDH mRNA and β-actin protein are used as the internal controls. LCM: the laser captured micro-dissection. In the study with mice specimens, mice Gapdh mRNA is used as the internal controls. *P < 0.05 for GIO group vs Control group.

Back to article page