Figure 6: Gibberellin responses in GoGID1 Arabidopsis transgenic lines.

(A) Germination assay to evaluate gibberellin sensitivity. The seeds of Arabidopsis transgenic lines (OE1 and OE37) and WT control plants were germinated on 1/2 MS culture medium with or without 40 μM uniconazole or a mixture of 40 μM uniconazole and different concentrations of GA4 (0.01 to 10 μM) for 5 days. Fifty seeds were used for each treatment. The data are shown as the means ± SD of biological triplicates for each treatment. (B) Seedling growth response to gebberellin. The seeds of transgenic lines (OE1 and OE37) and WT were germinated in Petri dishes on 1/2 MS culture medium containing 0.1 μM uniconazole (Uni). Seven days after germination, the seedlings were treated with 10 μM GA4 for 1 h and photographed 10 days later, bar: 1 cm. (C) Expression level assay of GA-response genes in transgenic lines (OE1 and OE37) and WT control. RNA was isolated from 3-week-old plants treated with 10 μM GA4 for 12 h and for perform qRT-PCR analysis. The data are shown as the means ± SD of biological triplicates.