Figure 5: Representative sequencing results.

Sequencing results of representative libraries prepared in duplicates for five methylated controls (0%, 25%, 50%, 75% and 100%) sequenced using primers generated and pooled using the PrimerSuite package using bisulfite multiplex PCR resequencing. (a) The number of reads per amplicon across each library in the individual pools. Read counts expressed in a log₁₀ scale. (b) Summary of methylation results of each library across the set of methylated controls. Level of methylation was maintained at a consistent level corresponding to the baseline level of methylation across each methylated control.