Table 1 Review of existing primer design programs.
From: PrimerSuite: A High-Throughput Web-Based Primer Design Program for Multiplex Bisulfite PCR
| Â | Multi-FASTA Input | Different Converted Bases | CpG adjustment | Homopolymer Assessment | Multiplex Pooling | Amplicon Size Adjustment | Selection between C-to-T or G-to-A converted genomes | Results Input | Dimer Predictions | Primers pairs @ | Reference | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Primer | Amplicon | |||||||||||
PrimerSuite | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Excel workbook | Yes | 215+ | This publication |
Bisearch | No | No | No | No | No | No | No | No | Online view | Yes | 19 | Aranyi et al.21 |
Bisulfite Primer Seeker* | No | No | No | No | No | No | No | No | No | 2 | zymoresearch.com | |
Epi-Designer # | Yes | No | No | Yes | Yes | No | Yes | Yes | Online view and excel export | No | 2 | epidesigner.com |
BisPrimer | No | Yes | No | No | No | No | No | No | Reported as plain text | No | 5 | Kovacova et al.10 |
Meth-Primer | No | Yes | No | Yes | Yes | No | Yes | No | Online view | No | 5 | Li et al.8 |
Kismeth | Yes | Yes | No | No | No | No | Yes | Yes | Online View | No | 3 | Gruntman et al.11 |
