Table 3 Summary of statistical mean using Wilxcon signed rank test.

From: PrimerSuite: A High-Throughput Web-Based Primer Design Program for Multiplex Bisulfite PCR

 

Single gel band

Multiple gel bands

p

Human genome#

Single end

464.78

4556.18

0.0005

Paired end

1.32

4.45

0.0022

Human repeats#

Single end

0.27

1.82

0.0004

Paired end

N/A*

N/A*

N/A*

  1. *Only one primer pair (C181 + C182) mapped 18 times in paired-end mode, producing amplicons varying in size between 29 bp to 219 bp (Fig. 3c); #Reference genomes were bisulfite converted prior to mapping.
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