Figure 4: SMAD4 and PTEN are direct transcriptional targets of miR-205 in OC cells. | Scientific Reports

Figure 4: SMAD4 and PTEN are direct transcriptional targets of miR-205 in OC cells.

From: Upregulation of MiR-205 transcriptionally suppresses SMAD4 and PTEN and contributes to human ovarian cancer progression

Figure 4

(A) Putative target sites for miR-205 in the 3′ UTR of SMAD4 and PTEN. Target sequences of SMAD4- and PTEN-3′ UTR were mutated. (B) Effects of miR-205 upregulation on endogenous SMAD4 and PTEN mRNA level which were analyzed by qRT-PCR. (C) Decreased protein level of SMAD4 and PTEN due to miR-205 overexpression in HO-8910 and SKOV-3 cells, GAPDH served as a loading control. (D) qRT- PCR and western blotting detection of PTEN expression in SKOV-3/DDP and SKOV-3 cells. (E) Analysis of luciferase activity. The 3′ UTR wild-type report plasmid or 3′ UTR mutant report plasmid of SMAD4 or PTEN were transfected into 293 T cells, and LV-miR-205 or LV-miR-Ctrl was co-transfected with each plasmid in 293 T cells. After which the luciferase activity was analyzed using the dual-luciferase report assay system. All the error bars indicate ± SEM. Statistical significance was concluded at *P < 0.05, **P < 0.01, ***P < 0.001.

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