Figure 2: Behavioural results for the 5 experimental groups: control, tumour, tum+FLX, tum+SSZ, tum+5-ASA/SP.

(A) In the sucrose preference test (SPT) preference is calculated as (sucrose water consumption/total fluid intake) × 100, where 50% represents equal consumption of sucrose water and regular water. Tumour mice showed a lower sucrose preference compared to control (P = 0.026). Treatments with FLX, SSZ, and 5-ASA/SP were associated with higher sucrose preference compared to untreated tumour mice (P = 0.040, P = 0.002, P = 0.035, respectively). (B) In the forced swim test (FST), tumour mice had higher immobility time compared to control mice (P = 0.033). FLX and SSZ were associated with lower immobility times compared to untreated tumour mice (P = 0.033, P = 0.019, respectively). (C) In the tail suspension test (TST), no significant changes were observed. Trends indicated a lower immobility time and higher log₁₀ PM associated with FLX and SSZ treatments compared to untreated mice. Data are presented as mean ± SEM. Results were analyzed using one-way ANOVA. The Holm-Šidák correction was used to correct for multiple comparisons. *P < 0.05, **P < 0.01.