Figure 3: DHCR24 could be induced by insulin stimulation via STAT3 in EC. | Scientific Reports

Figure 3: DHCR24 could be induced by insulin stimulation via STAT3 in EC.

From: Cholesterol Synthetase DHCR24 Induced by Insulin Aggravates Cancer Invasion and Progesterone Resistance in Endometrial Carcinoma

Figure 3

(A) DHCR24 DNA copy number amplification from the genomic-scale sequencing of EC in TCGA. (B) mRNA expression of DHCR24 stimulated by EGF, insulin, IL6, TGF-β and estradiol evaluated by qPCR. (C) The mRNA expression level of DHCR24 and STAT3 under STAT3 siRNA interference. (D) The protein level of DHCR24 was determined in ECC-1 and HEC-1A cells treated with insulin, GSK1904529A and Stattic by immunoblotting (cropped images are shown here, the non-cropped images are shown in Supplementary data 6). (E) A ChIP assay was performed to confirm the potential STAT3 binding site in the DHCR24 promoter region. IgG and input fraction were used as controls. (F) A ChIP assay was performed in two endometrial cancer cell lines, HEC-1A and ECC-1. (G) In total, 10 pairs of primers were constructed according to the promoter of DHCR24. (H and I) A Luciferase reporter assay was performed using ECC-1 and HEC-1A cells after transfecting the wild type plasmids and mutated plasmids (mutation site: red). The data shown are the mean ± SD. (*p < 0.05; **p < 0.01; ***p < 0.001).

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