Figure 2: ChA induces lytic cell death of M2 but hardly affects M1 viability.
From: Modulation of actin dynamics as potential macrophage subtype-targeting anti-tumour strategy
(A) F- and G-actin localization in polarized M0, M1 and M2. The staining was performed after cell fixation with Alexa Fluor 488 phalloidin (green, F-actin), Alexa Fluor 594 DNase I (red, G-actin), and DAPI (blue, nuclei); n = 3; scale bar, 10 μm. (B) Analysis of soluble (S100) and insoluble (P100) actin pools in macrophages after cell lysis, centrifugation (100,000 × g) and Western blot. Pictures are representative of three independent experiments. (C) MTT and (D) LDH assay of M0, M1 and M2 after incubation with ChA for 48 h (MTT) or 24 h (LDH). ChA was added 30 min prior to polarization. Data are expressed as percentage of (C) vehicle (0.1% DMSO) or (D) Triton X-100 (1%) controls, means + SEM; n = 4; *p < 0.05, **p < 0.01, ***p < 0.001 vs vehicle control.
