Figure 1: HCV infection suppresses DDX3, MTP expression and ApoB secretion. | Scientific Reports

Figure 1: HCV infection suppresses DDX3, MTP expression and ApoB secretion.

From: RNA helicase DDX3 maintains lipid homeostasis through upregulation of the microsomal triglyceride transfer protein by interacting with HNF4 and SHP

Figure 1

(a) HCV infection reduces the expression of DDX3 and MTP. The total cell extracts of HCV-infected/uninfected Huh-7.5.1 (see Materials and Methods) were collected and subjected to immunoblotting using anti-MTP (Santa Cruz Biotechnology), anti-DDX321 and anti-core (Abcam) antibodies, and GAPDH was used as a loading control. Intracellular protein levels were quantified by ImageJ, normalized to GAPDH and represented as amount relative to that of uninfected Huh-7.5.1 cells. (b) HCV infection leads to reduced secretion of ApoB. Culture media harvested from HCV-infected/uninfected Huh-7.5.1 cells were subjected to ApoB detection by ELISA (see Materials and Methods). Results are represented as means ± S.D. for at least three independent experiments and ***indicates p < 0.001, is considered to be significant.

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