Figure 7: DDX3 partially relieves the SHP-mediated repression effect on MTP promoter. | Scientific Reports

Figure 7: DDX3 partially relieves the SHP-mediated repression effect on MTP promoter.

From: RNA helicase DDX3 maintains lipid homeostasis through upregulation of the microsomal triglyceride transfer protein by interacting with HNF4 and SHP

Figure 7

HuH7 cells were transfected with pGL2/MTP-Luc(−611/+87) reporter plasmid (0.5 μg) alone or together with a combination of indicated amount of HA-HNF4, HA-SHP and HA-DDX3 expression plasmids. After 48 hr, cells were harvested and lucifearse activity assay was performed. The total amount of transfected plasmids was kept constant by adding control vector, pcDNA/HA. The relative luciferase activity is represented as activation fold compared to basal expression level of the MTP reporter plasmid alone (lane 1). Results are represented as the average (mean ± S.D.) of at least three independent experiments.

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