Figure 4: miR-199a-5p targets p27 and induces the G1-to-S cell-cycle transition.
From: MicroRNA-199a-5p promotes tumour growth by dual-targeting PIAS3 and p27 in human osteosarcoma
(A) TargetScan prediction software identified one seed match for miR-199a-5p in the 3′UTR of p27; the predicted seed-recognition site in the p27 mRNA sequence and the corresponding miR-199a-5p sequence are marked in red. (B) The relative luciferase activity of the p27 3′-UTR reporter plasmid was assayed in MNNG/HOS cells after transfection with pre/anti-miR-199a-5p or the corresponding control. (C) The relative level p27 mRNA in Saos-2 (left) and MNNG/HOS cells (right) after transfection with pre/anti-miR-199a-5p or the corresponding control. (D) The protein level of p27 in Saos-2 (left) or MNNG/HOS cells (right) after transfection with pre/anti-miR-199a-5p or the corresponding control. (E) The protein expression of p27 in the eight pairs of NAT and OS samples (left). Pearson’s correlation scatter plot comparing the fold changes in the expression of miR-199a-5p and the p27 protein in OS patients (right). (F) Cell cycle analysis of Saos-2 and MNNG/HOS cells after transfection with pre/anti-miR-199a-5p or the corresponding control. (G) Cell cycle analysis of MNNG/HOS cells after transfection with pre-scramble plus the PCI plasmid, pre-miR-199a-5p plus the PCI plasmid, pre-scramble plus the PCI-p27 plasmid or pre-miR-199a-5p plus the PCI-p27 plasmid for 48 hours. The data are expressed as the means ± SEM, **P < 0.01. NS: no significant change.
