Figure 6: Inhibition of TBK1 impairs DC activation induced by ΔN146 ex vivo.
From: An Engineered Herpesvirus Activates Dendritic Cells and Induces Protective Immunity
(A) Immature DCs were left untreated or treated with DMSO or TBK1 inhibitor BX795 (1 μM) for 1 h. Cells were then mock infected, infected by ΔN146 mutant (5 PFU/cell) or stimulated with LPS (500 ng/ml) in the presence or absence of BX795 (1 μM). At 12 h post-infection, cells were stained with CD11c and assayed for CD40 or CD86 positivity by flow cytometry. (B) DCs were treated as panel A and assayed by flow cytometry. Cells were gated on CD11c positive population and assayed for CD40 or CD86 fluorescence intensity.
