Figure 4: Validation of methyl-seq. | Scientific Reports

Figure 4: Validation of methyl-seq.

From: Genome-wide DNA methylation reprogramming in response to inorganic arsenic links inhibition of CTCF binding, DNMT expression and cellular transformation

Figure 4

Bisulfite conversion and pyrosequencing of specific gene promoter loci to confirm Methyl-MiniSeq data. (a) DNA methylation data from Methyl-MiniSeq study are shown using a UCSC browser track displaying our data set. High methylation in yellow and low methylation in red. (b) Graphical representation of methylation at each promoter or gene body sites. Lollipop representation of bisulfite converted DNA (NT on top iAs-T on bottom). PTPRJ (Chr11:48001742), OSBP2 (Chr22:31091206), NRG3 (Chr10:83634392), GIPC2 (Chr1:78511849). Schematic shows DNA hypo or hyper methylation in promotor region of representative genes and the impact of the methylation status on transcriptional activity. Arrow indicates the direction of differential methylation changes from normal (NT) cells to iAs-transformed (iAs-T) cells.

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