Figure 3: Correlation of drug-resistant gene mutations measured using the MALDI-TOF MS and culture-based drug susceptibility tests. | Scientific Reports

Figure 3: Correlation of drug-resistant gene mutations measured using the MALDI-TOF MS and culture-based drug susceptibility tests.

From: Rapid Sputum Multiplex Detection of the M. tuberculosis Complex (MTBC) and Resistance Mutations for Eight Antibiotics by Nucleotide MALDI-TOF MS

Figure 3

(A) Sixty-four clinical isolates with drug susceptibility test results were assessed for the drug-resistant gene mutation test using MALDI-TOF MS. Mutations were not identified in the all-sensitive drug isolates. Six isolates resistant to drugs did not exhibit a mutation using our testing panel. Mutations in the rpoB gene included five isolates at codon 516, one at codon 526, and 13 at codon 531; mutations in the katG gene were all at codon 315. Mutations in the inhA gene were all at the −15 nucleotide position, and mutations in the embB gene were all at codon 306. Mutations in the gene gyrA included two at codon 90 and five at codon 94, and mutations in the rpsL gene included one isolate at codon 88 and three at codon 43. (B–E) The mass spectrum of XDR-MTB (#14) (black solid line) overlapped with all-sensitive MTB (#9) (grey dotted line) and exhibited mutations in four genes, including rpoB (Ser531Leu) (B), katG (Ser315Thr) (C), embB (Met306Val) (D), and gyrA (Asp94Gly) (E).

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