Table 2 Comparison of Drug Resistant Gene Mutation Detection in Clinical Isolates by Sanger Sequencing and MALDI-TOF MS.

Sample ID	MALDI-TOF MS								Drug Susceptibility Test			Sanger Sequencing
Sample ID	rpoB	katG	embB	gyrA	gyrB	rpsL	inhA	pncA	Result	RIF	INH	Mutation for RIF	Mutation for INH
MTB-01	533	315							MDR	R	R	rpoB codon 533 CTG/CCG	katG codon 315 AGC/AAC
MTB-02	531		497			43	−15		MDR	R	R	rpoB codon 531 TCG/TTG	inhAr -15 C/T
MTB-03	531	315				43			MDR	R	R	rpoB codon 531 TCG/TTG	katG codon 315 AGC/AAC
MTB-04	522		306				−15		MDR	R	R	rpoB codon 522 TCG/TTG	inhAr -15 C/T
MTB-05	526								MDR	R	R	rpoB codon 526 CAC/TAC
MTB-06	526	315							MDR	R	R	rpoB codon 526 CAC/TAC	Kat G codon 315 AGC/ACC
MTB-07	513		406			43			MDR	R	R	rpoB codon 513 Q513P/L
MTB-08	531	315					−15		MDR	R	R	rpoB codon S531L	inhAr -15 C/T
MTB-09	526	315							MDR	R	R	rpoB codon H526D	katG codon 315 AGC/ACC
MTB-10	526	315							MDR	R	R	rpoB codon H526D	katG codon 315 AGC/ACC
MTB-11	531		306						MDR	R	R	rpoB codon S531L	INH: WT
MTB-12									N	S	S	rpoB: WT	INH: WT
MTB-13		315							MDR	R	R	rpoB: WT	katG codon 315 AGC/ACC
MTB-14		315							N	S	R	rpoB: WT	katG codon 315 AGC/ACC
MTB-15		315							N	S	R	rpoB: WT	katG codon 315 AGC/ACC
MTB-16	516		497						MDR	R	R	rpoB codon D516V
MTB-17	531	315	306				−15		MDR	R	R	rpoB codon S531L	inhAr -15 C/T
MTB-18	531								MDR	R	R	rpoB codon S531L
MTB-19	531	315							MDR	R	R	rpoB codon S531L	katG codon 315 AGC/ACC
MTB-20									N	S	S	rpoB: WT	ND

Quick links

Search