Figure 2: Comparison between the mouse ear sponge assay and the intradermal injection of tumor cells into mouse ears.
From: Modeling pre-metastatic lymphvascular niche in the mouse ear sponge assay
The B16F10Luc+ tumor cell suspension (5 × 105 cells/50 μl) was directly injected between the two ear skin layers of C57BL/6J mice (Intradermal Injection) or added on a cylindrical piece of sponge and then implanted in mouse ears (Sponge Implantation). (a) At different time points (Day 2, 4, 9 and 14) bioluminescence in mouse ears was recorded in vivo with a Xenogen system. (b) The draining sentinel LNs were dissected and their bioluminescence was visualized ex vivo over time. Numeric values displayed in the color scale for radiance are expressed in photons/sec/cm2. “L” represents left ears/LNs and “R” represents right ears/LNs, n = 8 sponges or LNs per group (4 mice/group).
