Figure 1: Transformation frequencies, foci formation and ComB expression in DNA repair mutants.
From: ComB proteins expression levels determine Helicobacter pylori competence capacity
(A) Transformation frequencies. The bar represents the median, the boxes display the inner quartile range and the whiskers represent the minimum and maximum values. Number of experiments and p value determined with Mann-Whitney U test are indicated in Table 1. (B) Microscopy images of fixed bacteria. For each of the indicated strains, merged images are presented with red for FMX64, blue for DAPI and green for ATTO488. Scale bars correspond to 5 μm. (C) Proportion of bacteria with foci. At least 3 independent experiments were analyzed and more than 1200 bacteria were counted for each strain. The percentage of bacteria harboring foci are indicated for the different strains. The bar represents the median, the boxes display the inner quartile range and the whiskers represent the minimum and maximum values. (D) Western blot of wild-type and different mutants against ComB10 (upper line) and ComB8 (lower line).
