Table 3 Effect of comB6-B10 operon overexpression on transformation frequency, foci formation and ComB8 expression.

From: ComB proteins expression levels determine Helicobacter pylori competence capacity

strain

Transformation a

Foci formation b

ComB expression c (relative value)

frequency (×10−4) median (min; max)

Relative value

Proportion, median (min; max)

Relative value

ComB8

ComB10

26695

3.9 (0.3; 26)

1

7.3 (4; 12)

1

1

1

pUreAComB6

15.0 (3; 45)

3,8****

17.1 (14; 23)

2.3***

2.6

3.0

  1. aTransformation frequencies were calculated as the number of StrR colonies per recipient CFU. ****p < 0.0001 with Mann-Whitney U test calculated between wt and the mutant.
  2. b3 independent experiments were analyzed and more than 1200 bacteria were counted for each strain. ***p = 0.0002 with Mann-Whitney U test calculated between wt and the mutant.
  3. cMedians of quantification from Western blots obtained with 3 independent strains are presented.
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