Figure 1: PrPC is present in mitochondria isolated from the brains of wild-type C57BL/6 and transgenic mice.
From: Cellular prion protein is present in mitochondria of healthy mice
(a) Top panel: PrPC is detectable in MACS purified mitochondria isolated from brain tissue of wild-type C57Bl/6 mice, transgenic mice overexpressing human PrPC (RM), overexpressing mouse PrPC containing the 3F4 epitope (Tg3F4), or mice underexpressing mouse PrPC without the GPI anchor (Tg44). Bottom panel: PrPC is detectable in highly purified MACS + Percoll mitochondrial preparations from which MAM have been removed. Each lane was loaded with 10 μg of MACS purified mitochondrial lysate or MACS + Percoll lysate. The rabbit monoclonal antibody EP1802Y, which recognizes a C-terminal epitope of PrPC, was used for detection. Molecular mass markers in kilodaltons are shown on the left. Each panel represents an individual blot. Blots were cropped for presentation purposes and to remove irrelevant or empty lanes. (b) Assessment of subcellular components in MACS purified and MACS + Percoll purified mitochondrial preparations. Markers used and predicted molecular weights: Na/K ATPase 112 kDa: plasma membrane, GRP78 78 kDa: endoplasmic reticulum, ATP5a 60 kDa: mitochondria, GAPDH 37 kDa: cytosol, Histone H3 17 kDa: Nucleus. Each panel represents an individual blot. BH = brain homogenate; mito = purified mitochondria. Molecular mass markers in kilodaltons are on the right. Each panel represents an individual blot.
