Figure 2: Mitochondrial PrPC is fully glycosylated.
From: Cellular prion protein is present in mitochondria of healthy mice
Brain homogenate (10 μg) or MACS purified mitochondrial lysate (10 μg) was loaded into each lane. The rabbit monoclonal antibody EP1802Y, which recognizes a C-terminal epitope of PrPC, was used for detection. BH = brain homogenate; mito = MACS purified mitochondria. Molecular mass markers are shown on the right. The arrow indicates full length unglycosylated PrPC while the asterisk indicates N-terminally truncated, unglycosylated PrPC. Each panel represents an individual blot. Blots were cropped for presentation purposes and to remove irrelevant or empty lanes.
