Figure 4: The N-terminus of fully glycosylated PrPC in mitoplasts is PK-sensitive.
From: Cellular prion protein is present in mitochondria of healthy mice
PK digestion of inner mitochondrial membrane fractions (mitoplast) leads to a PrPC band (arrow) which is not detected by the N-terminal PrP antibody SAF32. Following PNGaseF treatment, this band shifts to ~15 kD (asterisk). A non-PrP specific, cross-reactive band of approximately 36 kD is observed in all PNGaseF treated samples and may contribute to the intensity of the diglycosylated PrP bands (arrowhead). Molecular mass markers are shown on the right. Each panel represents an individual blot. Blots were cropped for presentation purposes and to remove irrelevant or empty lanes.
