Figure 1: Novel fluorescent reporters of CLK/CYC-mediated transcription and PER protein oscillations. | Scientific Reports

Figure 1: Novel fluorescent reporters of CLK/CYC-mediated transcription and PER protein oscillations.

From: Fluorescence circadian imaging reveals a PDF-dependent transcriptional regulation of the Drosophila molecular clock

Figure 1

(a) Transcriptional (3 × 69-VNP) and PER protein (per-TdT) reporters. (b) 3 × 69-VNP and PER-TDT expression in third instar larval brains dissected every 4 hr in LD cycles. Representative confocal images of the LNvs are shown. Clock neurons in the 3 × 69-VNP line were labelled by 1982clk-gal4, UAS-mCD8::RFP. The LNvs in the per-TdT flies were labelled by gal1118-GAL4, UAS-mCD8::VENUS. Scale bars are 5 μm. (c) Quantification of the reporter fluorescence intensity in clock neurons. Mean ± SEM. A.U., arbitrary unit. A minimum of 30 LNvs, 17 DN1s and 11 DN2s were analysed at each timepoint. (d) Nuclear/cytoplasmic fluorescence ratio of PER-TDT in adult and larval LNvs.

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