Figure 5: PDF cell-autonomously upregulates CLK/CYC-mediated transcription by an activity-dependent mechanism. | Scientific Reports

Figure 5: PDF cell-autonomously upregulates CLK/CYC-mediated transcription by an activity-dependent mechanism.

From: Fluorescence circadian imaging reveals a PDF-dependent transcriptional regulation of the Drosophila molecular clock

Figure 5

3 × 69-VNP expression in cultured clock neurons in response to various drug treatments. Graphs and heatmaps show the relative fluorescence intensity time course of single cells normalized to the value at t = 0. Dark red indicates values exceeding the uppermost colour scale of the heatmap. The red arrows indicate the time of drug application. (a) Wild-type clock neurons after the addition of PDF (20 μM) or vehicle at t = 0. (b) Clock neurons in pdfr mutants with PDF or vehicle treatment. (c) Wild-type clock neurons after the addition of TTX (100 nM) or GABA (10 μM), with or without PDF (20 μM) at t = 0. (d) Average 3 × 69-VNP fluorescence levels in wild-type clock neurons with the addition of different drugs. (e) Average 3 × 69-VNP levels in pdfr clock neurons treated with PDF or vehicle. Error bar, SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 by two-way ANOVA with Sidak’s correction for multiple comparisons.

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