Table 2 Characteristics of investigated STR loci: Ta (°C), annealing temperature; TD, touch-down PCR; F, forward; R, reverse; size range (bp); A, number of alleles; PID, probability that two individuals drawn at random share identical genotypes; PIDsib, probability of identity among siblings; repeated motif.
Locus | Label | Ta (°C) | Primer sequence (5′–3′) | Size-range (bp) | A | P ID | PIDsib | Repeated motif |
|---|---|---|---|---|---|---|---|---|
Lut435 | HEX | 48 | F: TGAAGCCCAGCTTGGTACTTC | 113–133 | 11 | 2.7 × 10−2 | 3.3 × 10−1 | (CA)15 |
R: ACAGACAGTATCCAAGGGACCTG | ||||||||
Lut615 | HEX | TD 52–48 | F: TGCAAAATTAGGCATTTCATTCC | 223–249 | 10 | 9.9 × 10−4 | 1.1 × 10−1 | (GT)12 |
R: ATTCTCTTTTGCCCTTTGCTTC | ||||||||
Lut818 | FAM | TD 52–48 | F: AAGGATGTGAAACAGCATTG | 142–184 | 8 | 4.5 × 10−5 | 3.9 × 10−2 | (GATA)3 |
R: CCATTTTATACACATAAATCGGAT | ||||||||
Lut457 | TET | TD 52–48 | F: CAGGTTTATGGCTTTATGGCTTTC | 153–175 | 8 | 2.1 × 10−6 | 1.4 × 10−2 | (CA)9 |
R: CAGGGTTTGATTTCTGGTGAGG | ||||||||
Lut701 | TET | TD 55–52 | F: GGAAACTGTTAAAGGAGCTCACC | 152–180 | 10 | 1.1 × 10−7 | 5.0 × 10−3 | (CCTT)2…(CTAT)9 |
R: CAGTGTTCATAAGGATGCTCCTAC | ||||||||
Lut453 | FAM | TD 52–48 | F: AGTGCTTTGTACTTGGTAATGG | 97–131 | 9 | 9.8 × 10−9 | 2.0 × 10−3 | (CA)9 |
R: AGACTGAAAGCTCTGTGAGGTC | ||||||||
OT19 | FAM | TD 55–52 | F: ATAGGTCTCTCAGCACGGTGTCT | 203–223 | 6 | 1.2 × 10−9 | 8.7 × 10−4 | (GGAA)6..(GAAA)7 |
R: TTAAATCCACATCTGTGACTCTGCA | ||||||||
Lut832 | TET | TD 52–48 | F: TGATACTTTCTACCCAGGTGTC | 176–192 | 5 | 1.6 × 10−10 | 3.8 × 10−4 | (GATA)8 |
R: TCCTTAGCATTATCTTATTTACCAC | ||||||||
Lut604 | FAM | TD 55–52 | F: TATGATCCTGGTAGATTAACTTTGTG | 97–109 | 6 | 2.8 × 10−11 | 1.9 × 10−4 | (GT)7 |
R: TTTCAACAATTCATGCTGGAAC | ||||||||
OT17 | HEX | TD 55–52 | F: ATCAGGTATGAGGATACATTTACCT | 144–162 | 4 | 6.9 × 10−12 | 1.0 × 10−4 | (CTTT)6 |
R: TGCAACCTACTTCTATATGAATTT |
