Table 4 Type, name and nucleotide sequence of mtDNA Cyt-b primers used in this study; *modified from Irwin et al.99.
Type | Name | Nucleotide sequence (5′-3′) |
|---|---|---|
Modern DNA: Lutrogale/Aonyx/Lutra | ||
Entire gene PCR | Lutra_L14724* | TGACTAGTAACATGAAAAATCACGTTG |
Lutra_H15915* | GGGATTCTGCATTTTTGGTTTACAAGAC | |
Semi-nested PCR and/or sequencing | LutroCb_fw583 | GTTCACCTCCTGTTTCTCC |
LutroCb_rev706 | AGAAGTAGGGCGCCCAGG | |
LutroCb_rev706_Aonyx | AGGAGTAGGGCGCCTAGG | |
LutroCb_fw298 | CGCGGCCTATACTATGGATC | |
LutroCb_rev417 | GATTACGGTTGCGCCTCAAAAG | |
LutroCb_fw775 | GCCAACCCGCTCAGTACACC | |
LutroCb_rev906 | GTGTGTAGCAGTGGGACGATG | |
Archival DNA: Lutrogale | ||
PCR and/or sequencing | LutroCb_fw583 | GTTCACCTCCTGTTTCTCC |
LutroCb_fw610 | GGCTCCAACAACCCCTCCGG | |
LutroCb_fw727 | GTACTATTCTCCCCAGACCT | |
LutroCb_rev746 | AGGTCTGGGGAGAATAGTAC | |
LutroCb_fw775 | GCCAACCCGCTCAGTACACC | |
LutroCb_rev794 | GGTGTACTGAGCGGGTTGGC | |
LutroCb_rev890 | GAYAAGATTAGGGCCAATAC | |
LutroCb_rev926 | GAGGTGTGTAGCAGTGGGACG | |
