Figure 2: Suppressive effects of DKK-1 on LPS-induced inflammatory response in bronchial epithelial cells.
From: LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974
BEAS-2B human bronchial epithelial cells were pretreated with 16–500 ng/ml of recombinant DKK-1 for 24 hours, followed by 0.1 μg/ml of LPS stimulation for 2 hours. Western blotting was used to assess protein levels and phosphorylation of NF-κB and IκB. Beta-actin and TBP were used as endogenous controls for total cell lysate and nuclear fraction, respectively (A). The binding of nuclear NF-κB for its target DNA sequence, 5′-GGGACTTTCC-3′, was measured by ELISA (B). The expressions of pro-inflammatory cytokine, IL-6 and IL-8, were measured by real time-qPCR (C, D). *p < 0.05, **p < 0.01, ***p < 0.001 compared with LPS-stimulated cells without DKK-1 pretreatment. ###p < 0.001 compared with cells without LPS stimulation.
