Figure 3: Synaptogenesis is maintained in early AP-2 depleted neurons.
From: Adaptor Protein 2 (AP-2) complex is essential for functional axogenesis in hippocampal neurons
(a) Representative images of axons expressing vGlut1-pHluorin after exposure to 50 mM NH4Cl (pH 7.4) to reveal intracellular pool of vesicles for control, AP-2KD and rescue conditions. Scale bar represent 5 μm. (b) Line scans of intensity from images in (a). (c) Mean values of the number of synaptic boutons per 20 μm of axon in Control, AP-2KD, and Rescue neurons. Control = 2.51 ± 0.06/20 μm (n = 6 cells), AP-2KD = 1.58 ± 0.07/20 μm (n = 5 cells), Rescue = 2.29216 ± 0.0956/20 μm (n = 6 cells). (d) Quantification of the total synaptic vesicle pool as measured by NH4Cl application in Control, AP-2KD, and Rescue neurons expressing vGlut1-pHlourin normalized to control cells. Control = 100.0 ± 14.8% (n = 8 cells), AP-2KD = 52.1 ± 9.2% (n = 5 cells), Rescue = 87.2 ± 1.8% (n = 5 cells). ***p < 0.001. One-way ANOVA.
