Figure 4: Activity-driven Ca2+ influx and vesicle fusion are impaired in early AP-2 depleted synapses.
From: Adaptor Protein 2 (AP-2) complex is essential for functional axogenesis in hippocampal neurons
(a) Representative single action potential stimulated GCaMP6f traces in control (black) and early AP-2KD neurons (red). (b) Mean value of GCaMP6f 1AP response amplitudes in control and AP-2KD neuron. Con = 0.0302 ± 0.71 (n = 7 cells), AP-2KD = 0.0021 ± 0.06 (n = 8 cells). (c) Representative vG-pH trace of neuron in control and early AP-2KD neurons. Neurons expressing vG-pH only, vG-pH with shRNA against AP-2 were stimulated with 100 action potential at 10 Hz. (Inset) ΔF image of synaptic bouton after 100 action potential at 10 Hz. (d) Mean values of the amount of synaptic transmission by 100 AP. Con = 8.69 ± 1.24% (n = 8 cells), AP-2KD = 0.65 ± 0.38% (n = 7 cells). ***p < 0.001.
